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. 2019 Dec 30;9(1):88. doi: 10.3390/cells9010088

Figure 7.

Figure 7

hCPs seeded on silicon slide maintain their neural immature identity. Immunostaining of hCPs for NESTIN (green), SOX2 (red) and nuclear staining with Hoechst (blue) in 3D culture (A) and 2D culture (B) cultured for 14 days. (C) Quantitative RT-PCR assay showing the expression levels of Nestin and β3-Tubulin transcripts in hCPs grown in 2D or 3D cultures. Scale bar: 100 μm (A) and 50 μm (B). p < 0.01 (**), not significant (ns).