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. 2019 Dec 30;9(1):88. doi: 10.3390/cells9010088

Figure 8.

Figure 8

hCPs seeded on silicon micropillar arrays preserve their cortical regional identity and upon exposure to differentiative conditions, generate cortical glutamatergic neurons. (A) eGFP+ve hCPs cultured for 14 days on 3D silicon devices preserve the expression of the cortical progenitor marker TBR2 (red). Nuclei are stained with Hoechst (blue). Scale bar: 50 μm. (B) hCPs cultured for 5 days on 3D silicon devices and then exposed for 35 days to differentiative conditions maturate into cortical glutamatergic neurons. Left: cultures stained for the pan-neuronal neuronal marker β3-TUBULIN (red). Nuclei are stained with Hoechst (blue). Scale bar: 10 μm. Right: cultures stained for the mature neuronal marker MAP2 (red) and for the cortical neuronal marker CUX1 (green). Nuclei are stained with Hoechst (blue). Scale bar: 10 μm.