Figure 7.

Effects of the combined treatment on adherent malignant B16-F10 melanoma cells using µsPEF at 0, 500, and 1400 V/cm. (a) Percentage of electropermeabilised cells and (b) intracellular fluorescence of YO-PRO^®-1 iodide entering the cells as a function of the seven combined protocols applied. Data are presented as mean (for a) and median (for b) values ± SD of independent triplicates. Statistical differences were analysed using One-way ANOVA followed by Bonferroni’s multiple comparison test. * p < 0.05, ** p < 0.01, ***, p < 0.001, and **** p < 0.0001 significant differences.