Table 1.
Primary and secondary antibodies used in IB, IF, ChIP.
| Antigen | Manufacturer | Species, Type | Catalog Number | Dilution |
|---|---|---|---|---|
| DKK1 | Santa Cruz/TX, USA | Mouse monoclonal | sc-374574 | 1:1000 |
| DKK1 | Cell Signaling/MA, USA | Rabbit monoclonal | 48367 | 1:1000 |
| C/EBPβ | Santa Cruz/TX, USA | Mouse monoclonal | sc-7962 | 1:1000 |
| VDR | Cell Signaling/MA, USA | Rabbit monoclonal | 12550 | 1:1000 |
| Active β-catenin | Cell Signaling/MA, USA | Rabbit monoclonal | 19807 | 1:1000 |
| β-catenin | Cell Signaling/MA, USA | Rabbit polyclonal | 9562 | 1:1000 |
| RUNX2 | Cell Signaling/MA, USA | Rabbit monoclonal | 12556 | 1:1000 |
| RUNX2 | Santa Cruz/TX, USA | Mouse monoclonal | sc-101145 | 1:1000 |
| Osteonectin, ON | Santa Cruz/TX, USA | Mouse monoclonal | sc-73472 | 1:1000 |
| Osteopontin, OPN | R&D System/MN, USA | Goat polyclonal | AF1433 | 1:2000 |
| Osteocalcin, OCN | Santa Cruz/TX, USA | Mouse monoclonal | sc-365797 | 1:1000 |
| Osteocalcin, OCN | Abcam/Cambridge, UK | Mouse monoclonal | ab13420 | 1:1000 |
| IgG | Millipore/MA, USA | Rabbit monoclonal | pp64 | 1:10000 |
| β-actin | Cell Signaling/MA, USA | Rabbit monoclonal | 4970 | 1:10000 |
| β-actin | Cell Signaling/MA, USA | Mouse monoclonal | 3700 | 1:10000 |
| GAPDH | Cell Signaling/MA, USA | Rabbit monoclonal | 2118 | 1:10000 |
| Alexa-488 | ThermoFisher/MA, USA | Mouse | A11001 | 1:100 |
| Cy3 | ThermoFisher/MA, USA | Rabbit | A10520 | 1:100 |
| HRP | Jackson ImmunoResearch/CA, USA | Mouse | 115-035-003 | 1:2000 |
| HRP | Jackson ImmunoResearch/CA, USA | Rabbit | 111-035-003 | 1:2000 |