Figure 6.

Sequence comparison of the hypervariable region of the Ras isoforms and the generated Ras mutants. (A) Sequence comparison of the hypervariable region of K-Ras, H-Ras, and N-Ras. The farnesylated cysteine is marked in green. (B) Overview of the Ras mutants in which the two lysines of K-Ras (bold) or corresponding amino acids in H-Ras and N-Ras, respectively, were exchanged. Substitutions are shown in red. (C) Overview of the K-Ras mutants in which the polybasic domain was exchanged by the corresponding amino acids of H-Ras or N-Ras. (B,C) The interaction of the Ras proteins with Galectin-8, based on the experiments shown in Figure 6; Figure 7, is summarized, as follows: [+++] = strong interaction, [+] = weak interaction [−] = no interaction.