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hrOSM dependent induction of migration relies on intracellular generation of ROS. Flow citometry analysis to evaluate ROS intracellular generation in LX2 cells untreated or treated for 1 h with hrOSM 10 ng/mL or with H2O2 100 µM for 15 min, used as positive control, or pre-treated for 1 h with the pharmacological inhibitor of NADPH oxidase (APO: apocynin 250 µM) and then exposed to hrOSM 10 ng/mL for 1 h. * p < 0.05, ** p < 0.01 versus control value, # p < 0.05 versus OSM value.
