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. 2019 Dec 20;9(1):28. doi: 10.3390/cells9010028

Figure 10.

Figure 10

(A–F) Involvement of OSM-dependent intracellular ROS generation in the activation of specific signal pathways. Western blotting analysis of phosphorylated levels of ERK 1/2 (A,B), JNK 1/2 (C,D) and c-Akt (E,F) in LX2 cells exposed to hrOSM 10 ng/mL for 15 and 30 min or, where indicated, pre-treated 1 h with the pharmacological inhibitor of the NADPH oxidase (APO: apocynin 250 µM) and then exposed to hrOSM 10 ng/mL. In some experiments LX2 cells were treated with the inhibitor apocynin alone for 1 h (B,D,F) and incubated for additional 30 min. Equal loading was confirmed by re-probing the same membrane with un-phosphorylated protein.