Figure 11.

(A–C) Involvement of intracellular generation of ROS in the OSM-dependent recruitment/ stabilization of HIF1α. Quantitative real time PCR (q-PCR) analysis of HIF1α transcript levels in LX2 exposed to hrOSM 10 ng/mL up to 24 h (A) or in cells pre-treated with the pharmacological inhibitor of the NADPH oxidase (APO: apocynin 250 µM) or with the JAK2 inhibitor (AG490 100 µM) for 1 h and then exposed or not to OSM 10 ng/mL for 6 h (times in which HIF1α transcript levels were more significant). (B) Data are expressed as means ± SEM of three independent experiments. * p < 0.05, ** p < 0.01 versus control value, # p < 0.05; versus OSM value. (C) Western blotting analysis of HIF1α protein levels in LX2 untreated or treated with hrOSM 10 ng/mL (from 15 min to 24 h). Equal loading was confirmed by re-probing the same membrane with β-action.