Figure 14.

Intracellular ROS involvement in modulation of LX2 cells pro-inflammatory response. Quantitative real time PCR (q-PCR) analysis of CCL2 transcript levels in LX2 exposed to hrOSM 10 ng/mL for different experimental times. In some experimental conditions LX2 cells were pre-treated for l h with the pharmacological inhibitor of the NADPH oxidase (APO: apocynin 250 µM) or with the JAK2 inhibitor (AG490 100pM) and then exposed or not to hrOSM 10 ng/mL. Data are expressed as means ± SEM of three independent experiments. ** p < 0.01 versus control value, # p < 0.05, ## p < 0.01 versus OSM value.