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. 2020 Jan 4;9(1):121. doi: 10.3390/cells9010121

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibition of mitochondrial fusion affects Oxygen Consumption Rate and ATP production. (A) Real-time analysis of basal Oxygen Consumption Rate (OCR) in Huh7 shOPA1 and shMFN1 cells (n = 3). (B) ATP production of Huh7 cells with OPA1/MFN1 downregulation was reduced compared with control group (n = 6). (C) Real-time analysis of basal OCR in CCA organoids with shOPA1 and shMFN1 (n = 3). (D) ATP production of CCA tumor organoids with OPA1/MFN1 downregulation was reduced compared with control group (n = 6). Histograms show means ± SEM with p value derived by the Mann Whitney test.