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. 2020 Jan 6;9(1):132. doi: 10.3390/cells9010132

Figure 1.

Figure 1

SUN1 or SUN2 depletion induces actin cap reorganisation. Western blotting (WB) of (A) SUN1- and (B) SUN2-depleted vascular smooth muscle cells (VSMC) lysates. Confocal immunofluorescence analysis imaging the apical and middle sections of F-actin (red), SUN1 or SUN2 (green), and DAPI (blue) stained (C) control and SUN1-depleted and (D) control and SUN2-depleted VSMCs grown on 12 kPa hydrogels. Graphs show (E) number of VSMC possessing actin caps and (F) the number of VSMCs possessing bright or faint actin cap staining displayed by control, SUN1- and SUN2-depleted VSMCs grown on 12 kPa hydrogels. Graphs represent the combined data from three independent experiments analysing >20 cells (*** p < 0.0001).