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. 2020 Jan 2;12(1):121. doi: 10.3390/cancers12010121

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Time window of vascular changes after 2 × 12 Gy in LLC tumors. (A) Fractionation and collection schedule. (B) Immunohistological staining for hypoxia (pimonidazole, B), vessels and perfusion (CD31 and Hoechst 33342, C) in function of time after RT initiation in LLC tumors. (D–F) Quantification of hypoxia (pimonidazole, D), perfusion (Hoechst 33342, E) and vessel density (F) after RT initiation in LLC tumors. Controls were collected at d9. Images and analysis represent ≥7 tumors per point. * indicates p < 0.05.