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. 2019 Dec 19;9(1):18. doi: 10.3390/cells9010018

Figure 2.

Figure 2

Activation of the NFAT pathway by PEP/calcium. NFAT is activated by ionomycin (1 µM) and inhibited by CsA (1 µM) (A). NFAT activity at various glucose concentrations in the culture media (B). NFAT activation levels at different extracellular PEP loads (C). Direct immunofluorescence for NFATc1 (green) and nuclear associated DAPI (blue) in basal, ionomycin (1 µM) and CsA (1 µM) conditions (D). Direct immunofluorescence for NFATc1 (green) and nuclear DAPI (blue) in the presence or absence of glucose in the culture media (E). NFAT target genes PTGS2 and IL-6 mRNA levels in the presence of ionomycin (1 µM) or ionomycin plus CsA (1 µM) (F,I). NFAT target genes PTGS2 and IL-6 mRNA levels at various glucose concentrations in the culture media (G,J). NFAT target genes PTGS2 and IL-6 mRNA levels at different extracellular PEP loads (H,K). Data are the mean ± SE, n = 5. One-way ANOVA, with Sidak post-test, * p < 0.05, ** p < 0.01, *** p < 0.001. * versus basal, 0 mM glucose or 0 mM extracellular PEP (A–C; F–K). Scale bar represents 20 μm.