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Interaction and uptake of OMVs and liposomes after incubation with A549 cells. The percentage of positive cells (which interacted with DiI-labelled OMVs) after (a) 4 h and (d) 24 h of incubation. The MFI after (b) 4 h and (e) 24 h of incubation. Representative histograms of Cbfe23 OMVs (green), Cbv34 OMVs (orange), liposomes (blue) and untreated control (red) after (c) 4 h and (f) 24 h of incubation. Mean ± SEM, n = 3. * p < 0.05, ** p < 0.005, *** p < 0.0005 and **** p < 0.0001.
