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Death receptor dependent markers were markedly increased by magnolol combined with sorafenib as compared to alone treatment. SK-Hep1 cells treated with magnolol (50 µM) or LY294002 (10 µM) combined with sorafenib (10 µM) were stained for (A,B) FAS (C,D) FASL and evaluate the activation with flow cytometry. (E,F) Cleaved caspase-8 activation was evaluated by flow cytometry. (** p < 0.01 was compared with 0 µM, ## p < 0.01 were both compared with alone treatment).
