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. 2019 Dec 23;11(1):18. doi: 10.3390/genes11010018

Figure 3.

Figure 3

Vulpinic acid induces osteogenesis via H3 acetylation in 10T1/2 MSCs. (A) Schematic representation of BMPs-mediated osteogenic or adipogenic commitment of MSCs. (B) Immunoblot analysis of 10T1/2 cells treated with vulpinic acid (10 or 40 μM) for 24 h in the presence of BMP2 or BMP4. (C) 10T1/2 cells were treated with or without vulpinic acid (40 μM) for 24 h in the presence of BMP2 or BMP4. ChIP assay was performed with IgG and acetylated H3 antibodies followed by real time PCR with primers for promoter region of Runx2 gene. (D) The mRNA levels of the Bmp2, Ocn, and Sp7 genes in 10T1/2 cells treated with vulpinic acid (10 or 40 μM) for 24 h. (E) The mRNA levels of Cebpa and PPARγ genes in 10T1/2 cells treated with vulpinic acid (10 or 40 μM) for 24 h in the presence of BMP4. Data represent means ± SEM for n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001.