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. 2019 Dec 23;11(1):18. doi: 10.3390/genes11010018

Figure 4.

Figure 4

Vulpinic acid promotes adipogenesis from 3T3-L1 preadipocytes. (A) Schematic representation of adipogenesis process. 3T3-L1 cells were incubated with an adipogenic medium in the absence or presence of vulpinic acid. (B) The mRNA levels of Adipsin, Fabp4, and PPARγ genes in 3T3-L1 adipocytes incubated with vulpinic acid (10 or 40 μM) during adipogenesis. (C) The mRNA levels of Adipoq and Leptin genes in 3T3-L1 adipocytes incubated with vulpinic acid (10 or 40 μM) during adipogenesis. (D) Immunoblot analysis of 3T3-L1 adipocytes incubated with vulpinic acid (10 or 40 μM) during adipogenesis. Data represent means ± SEM for n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001.