Figure 4.

IF1-silenced colon cancer cells have more aggressive phenotype. Cellular proliferation in shIF1 (red lines and bars) and IF1 (green lines and bars) was assessed by protein concentration (n = 3; A) and by the incorporation of EdU (n = 6; B) into cellular DNA. (C) Rates of basal, oligomycin sensitive and maximum respiration in shIF1 and IF1 cells determined in the X24 Seahorse Flux Analyzer (n = 3). OL, oligomycin; DNP, dinitrophenol; R + A, rotenone + antimycin A. (D) Time frames of the wound-healing assay and its quantification (n = 3). Magnification 4x. (E) Anchorage-independent growth in soft-agar and its quantification (n = 4). Magnification 10x. (F) Cell death determination by flow cytometry using Annexin V staining after overnight treatment with 0.25 µM staurosporine (STS) and 60 µM hydrogen peroxide (H₂O₂) (n = 5). (A–F) Results shown are means ± S.E.M; *, p ≤ 0.05 by Student’s t test.