Figure 5.

In vivo tumorigenesis and metastasis assays. IF1-silenced and overexpressing HCT116 cells were injected (A–C) into the left and right flank of sixteen mice, respectively or (D) into the tail vein of seven mice per condition. (A) Shows bioluminescence imaging of luciferase-positive HCT116 tumors 4 weeks after injection. The graphs show the bioluminescence (B) and tumor volume (C) of shIF1- and IF1-tumors. (D) Bioluminescence imaging of lung metastasis in mice injected with IF1 and shIF1 cells. The histograms show the percentage of mice with metastases, the number of metastases and its bioluminescence. Results shown are means ± S.E.M. IF1, IF1-overexpressing cells are represented by green bars and shIF1, IF1-silencing cells by red bars.