Figure 2.

Additive decrease of metabolic activity using cisplatin or gemcitabine with physical plasma-conditioned Ringer’s lactate in pancreatic cancer cells. (a) Experimental procedure of the in vitro treatment regimens with the application of different treatment liquids (30 min exposure time; CTx = chemotherapeutic agents, plasma = physical-plasma-conditioned Ringer’s lactate, RiLac = Ringer’s lactate before the solutions were replaced with fresh cell culture medium) at 0 h and 24 h, as well as downstream analysis at 48 h; (b,c) the metabolic activity at 48 h of (b) MiaPaca and (c) PaTuS pancreatic cancer cells; (d) percent of the amount of H₂O₂ initially induced through the plasma treatment in the combinational regimen and post-exposure to plasma-conditioned RiLac. Data (b,c) are representatives out of eight independent experiments, or (d) representatives showing mean +SD. Statistical significance was calculated utilizing ANOVA.