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. 2020 Jan 2;12(1):123. doi: 10.3390/cancers12010123

Figure 7.

Figure 7

The combination of cisplatin with physical plasma-conditioned Ringer’s lactate showed additive toxicity in three-dimensional tumors grown on chicken embryos. (a) Schematic overview of in ovo experiments and image of treatment with liquids of three-dimensional pancreatic tumors grown on the chorion-allantois membrane of fertilized chicken eggs (TUM-CAM); (b,c) tumor weight quantified at 48 h post-treatment with Ringer’s lactate (RiLac), plasma-conditioned RiLac, cisplatin, or the combination of physical plasma-conditioned RiLac and cisplatin in MiaPaca (b) and PaTuS (c) tumors; (d,g) representative images of apoptotic MiaPaca (d) and PaTuS (g) cells (blue = Draq5+, red = TUNEL+, green = Ki67+; scale-bar = 100 µm) in cryo-sections of the tumors exposed to the combination treatment (I), cisplatin (II), plasma-RiLac (III) and RiLac alone (IV); (e,h) algorithm-based quantitative image analysis of the percentage of apoptotic cells (TUNEL+/Draq5+) in MiaPaca (e) and PaTuS (h) tumors; (f,i) penetration depth of the treatment regimen in MiaPaca (f) and PaTuS (i) tumors. Data are representative from two independent experiments with eight to thirteen eggs per group and show (b,c) individual values with their mean ± SD, and (e,f,h,i) median and min to max. Statistical significance was calculated utilizing ANOVA.