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eIFsixty-i compounds do not alter nucleolar integrity. Representative images of eIF6 and NPM-stained nucleoli from H226 cells in the absence (control) or presence of eIFsixty-i compounds (eIFsixty-1, eIFsixty-4, and eIFsixty-6). Cells were treated with eIFsixty-i compounds for 1 h at the IC50 concentration. Actin and nuclei were visualized by incubating fixed cells with phalloidin or DAPI (4′,6-diamidino-2-phenylindole), respectively (scaling bars, 10 µm).
