Figure 2.

SKF96365 increases NMDA-induced Ca²⁺ levels. (a) Analysis of the [Ca²⁺]_i induced by NMDA (100 μM) and glycine (10 μM) in the presence of 5 μM nimodipine, 30 μM CNQX, and 50 μM MM; 50 μM D-AP5 or 30 μM SKF96365 (SKF) based on ratiometric measurements with Fura2-AM. F₃₄₀/F₃₈₀ values just before adding the NMDAR agonist were normalized to the same values (1). The data represent n independent experiments that were conducted on four different primary cultures corresponding to 516 (NMDA, n = 13), 305 (NMDA-MM, n = 10), 245 (NMDA-DAP, n = 10) and 624 (NMDA-SKF, n = 19) analyzed cells. (b) Summary of data from (a) shown as area under the curve (AUC), which was calculated from the moment immediately before the addition of NMDA. ** p < 0.01; *** p < 0.001 significantly different compared with NMDA (ANOVA followed by Tukey’s Multiple Comparison Test).