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. 2020 Jan 6;9(1):128. doi: 10.3390/cells9010128

Figure 3.

Figure 3

Effect of db-cAMP alone or in combination with IL-4 on STAT3 signaling and Arginase-1 expression. BMDMs from BALB/c mice were treated with db-cAMP (100 µM) for 5, 10 and 30 min (A) or were stimulated with IL-4 (5 ng/mL), db-cAMP (100 µM) or db-cAMP (100 µM) + IL-4 (5 ng/mL) for 30 min (B). Cell lysates were subjected to western blot analysis to assess pSTAT3 (A,B). In B, two exposures of p-STAT3 are presented to show the full range of expression. β-actin was used as a loading control. Densitometry analyses are shown (A,B). In (C) BMDMs were untreated or stimulated with db-cAMP (100 µM), IL-4 (20 ng/mL) or db-cAMP (100 µM) + IL-4 (20 ng/mL) for 6 h, and analyzed by qPCR for the expression of Arginase-1. Results are expressed as fold increase (qPCR) and are shown as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 when comparing with untreated (UT) BMDMs.