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. 2020 Jan 10;12(1):181. doi: 10.3390/cancers12010181

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ire cells exhibited higher mitochondrial bioenergetics. PE089 and Ire cells were treated with or without 100 ng/mL EGF and 1 μM gefitinib for 24 h. The time-course progress curves of the oxygen consumption rate (OCR) are shown in (A) the control cells, (B) the EGF-treated cells, (C) gefitinib-treated cells, and (D) combined treatment with EGF and gefitinib. Individual parameters for basal respiration (E), spare capacity (F), ATP production (G), and maximal respiration (H) are represented. The values represent the mean ± S.D. from three independent experiments and each experiment performed in duplicate. The presence of the same letters on the bars indicates no significant difference (p > 0.05) between the two different conditions and the presence of different letters on the bars indicates a significant difference (p < 0.05) between the two different conditions.