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. 2020 Jan 10;12(1):181. doi: 10.3390/cancers12010181

Figure 7.

Figure 7

AZD3965 exhibited inhibitory effects on the cell growth and mitochondrial bioenergetics of Ire cells. (A) Dose-dependent cell viability assay. Reduction in cell viability was assessed in PE089 cells and Ire cells after different concentrations of AZD3965 in 2% FBS medium. The star signs showed significant differences compared to a control group for each cell. (B) The proliferation of PE089 and Ire cells in response to 1 μM AZD3965 for 0, 24, 48 and 72 h was measured by the trypan blue-dye exclusion method. (C) The migratory ability of PE089 and Ire cells in response to 1 μM AZD3965 was determined by a migration assay. Cell images were taken at 0 and 18 h, and the migration area was measured by ImageJ. Significant inhibition by AZD3965 of cell proliferation, cell survival, and cell motility were observed in both PE089 and Ire cells. (D) The effects of AZD3965 on the mitochondrial bioenergetics of PE089 and Ire cells were examined. Individual parameters of OCR for basal respiration, spare capacity, ATP production and maximal respiration were analyzed by XF24 extracellular flux analyzer. The values represent the mean ± S.D. from three independent experiments and each experiment performed in duplicate. * p < 0.05. ** p < 0.01. *** p < 0.001.