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. 2019 Dec 18;9(1):3. doi: 10.3390/cells9010003

Figure 4.

Figure 4

PlexinB1 knockdown affects the level of glucocorticoid-responsive gene products. PC3 and DU145 cells were transfected with two different siRNAs to PlexinB1 or non-silencing siRNA(control) and then grown in medium with 10%FCS (which contains corticosteroids to stimulate GR activation). Protein levels of PlexinB1 (control) (a), FKBP51 (b), GILZ (c) and GR (d) were detected by immunoblotting. (e) PC3 or DUI45 cells were treated with dexamethasone (10 nM) and protein levels of FKBP51, GILZ, and GR were detected by immunoblotting. Bar charts show band intensities relative to actin (n = 3). Error bars denote SEM, * p < 0.05, Ttest.