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. 2019 Dec 18;9(1):3. doi: 10.3390/cells9010003

Figure 6.

Figure 6

Knockdown of PlexinB1 expression antagonises the reduction in cell migration and cell size induced by dexamethasone. (a) Cell migration. (i). PC3 cells were transfected with non-silencing siRNA (NS) or siRNA to PlexinB1 and knockdown of expression detected by immunoblotting (representative image). (ii) Cell motility was assessed in transwell assays in the absence or presence of dexamethasone (10 nM) and the ratio of cells migrated in the presence/absence of dexamethasone (Dex) calculated. Error bars denote SEM, * p < 0.05, Ttest (iii). Representative images of migrated cells in transwell assay. (b) Cell area. (i). PC3 cells plated on coverslips were transfected with non-silencing siRNA (NS) or siRNA to PlexinB1. After 72 h, the cells were treated with dexamethasone for 30 min, fixed and stained for GR (Alexa488, green), actin (phalloidin 555 red) and DAPI (blue). Cell area was calculated using ImageJ and the ratio of cell area in the presence /absence of dexamethasone calculated. Error bars denote SEM, * p < 0.05, Ttest, (ii). Representative images of cells with and without dexamethasone treatment.