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. 2020 Jan 17;9(1):238. doi: 10.3390/cells9010238

Figure 1.

Figure 1

A high gene expression profile consisting of 70 genes associated with a functional aneuploidy (CIN70) score is negatively associated with disease-specific survival in luminal A (LumA) and triple-negative breast cancer (TNBC) subtypes and correlates with the expression of RAD51 and CHK1. (A–C). Kaplan–Meier analysis of the CIN70 score as prognostic factor for disease-specific survival (DSS) in breast cancer patients (A) (n = 400) patients with LumA (B) (n = 200) and TNBCs (C) (n = 150) using the two extreme quartiles. The CIN70 score defines the differential mRNA expression of 70 genes in tumors classified as stable and unstable based on their structural and numerical chromosomal alterations [3]. DSS is plotted against time after therapy. All, LumA and TNBC tumors with CIN high showed significantly worse 5- and 10-year DSS compared to tumors with CIN low, with 52% vs. 86% after 10 years for all, 84% vs. 67% after 10 years (n.s/p = 0.0084) for LumA and 52% vs. 62% after 10 years (n.s/n.s) for TNBC tumors. p-values were calculated on the basis of the log-rank test. (D,E). mRNA expression of RAD51 (D) and CHK1 (E) for LumA and TNBC tumors of the Metabric data set sorted by CIN70 score using the two extreme quartiles. RAD51 is expressed significantly higher in CIN high than in CIN low tumors, both in LumA, with −0.88 ± 0.09 vs. 0.27 ± 0.1 and −0.11 ± 0.01 vs. 1.92 ± 0.19, respectively. A significantly increased expression in CIN high compared to CIN low was also found for CHK1, with 1.17 ± 0.04 and −0.03 ± 0.096 for LumA and 0.13 ± 0.1 vs. 2.08 ± 0.1 for TNBC. (F,G). Kaplan–Meier analysis of DSS of 400 patients according to the RAD51 (F) or CHK1 (G) expression using the two extreme quartiles. DSS is plotted against time after therapy. Patients whose tumor had a high expression of RAD51 showed significantly lower DSS compared to low RAD51 expression (68% vs. 62%). For the expression of CHK1, the negative effect of a high expression on survival was even more evident (62% vs. 80%). p-values were calculated on the basis of the log-rank test (**** p < 0.0001; Student’s t-test).