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. 2019 Dec 24;12(1):57. doi: 10.3390/cancers12010057

Figure 4.

Figure 4

Constitutively active protein kinase B (AKT) abolished the inhibition of programmed death-ligand 1 (PD-L1) and caspase 3 expression which were reversed by Salmonella (S.C.). Tumor cells were seeded in 6-well plates and transfected with constitutively active protein kinase B (AKT) for 16 hours and then treated with Salmonella 100 multiplicity of infection (M.O.I.) for 90 min. Subsequently, cells were cultured in free DMEM with 1% Gentamicin for 24 h. (A) Quantified band intensities of respective proteins of B16F10 and LL2 cells were lysed and the protein kinase B (AKT)/mammalian targets of rapamycin (mTOR)/ p70 ribosomal s6 kinase (p70s6K) and PD-L1 markers were analyzed. (B) Quantified band intensities of respective proteins of EL4 cells were co-cultured with Salmonella-treated tumor cells for 24 h, and the caspase 3 (17/19 kDa) expression was analyzed. (For Western blot analysis, please see Supplementary Figure S4) The inserted values indicate protein expression compared to β-actin. Mean ± Standard Deviation (SD) represented independent expression. Statistical significance was calculated using Student’s t-test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.