Table 1.
Optimized ChIP-Seq experimental procedures for each tissue.
| Parameter | Adipose | Brain | Heart | Lamina | Liver | Lung | Muscle | Ovary |
|---|---|---|---|---|---|---|---|---|
| Starting Tissue (mg) | 220 | 90 | 105 | 100 | 40 | 40 | 100 | 85 |
| Homogenization Time (min) | 8 | 5 | 5 | 9 | n/a | 5 | 5 | 5 |
| Duration Fixed (min) | 10 | 9 | 9 | 9 | 9 | 9 | 9 | 9 |
| Fixation Temp. (°C) | 37 | 23 | 23 | 23 | 23 | 23 | 23 | 23 |
| Shearing Volume (uL) | 400 | 1500 | 1800 | 1800 | 1500 | 1500 | 1800 | 1800 |
| Shearing Cycles | 5 × 8 1 | 10 | 13 | 10 | 12 | 10 | 12 | 10 |
| Chromatin per IP (ng) | 700 | 300 | 500 | 700 | 450 | 800 | 260 | 1200 |
1 Samples were sonicated for five sets of eight cycles.