Table 5.
Top five significantly enriched transcription factor binding motifs identified in tissue-specific active enhancers. Tissue specificity of the active enhancers was defined by overlap of H3K4me1 and H3K27ac in the same tissue and no overlap of these marks in this region in any other tissues, and tissue specificity of the binding motifs was then defined by detection of an enriched binding motif in only one tissue.
| Rank | Motif ID | Consensus | Adjusted p-Value | UniProt Entry |
|---|---|---|---|---|
| Adipose | ||||
| 1 | SP3 | VCCACGCCCMC | 1.49 × 10−10 | Q02447 |
| 2 | TFDP1 | VSGCGGGAAVN | 1.74 × 10−10 | Q14186 |
| 3 | TFAP2A | HGCCYSAGGCD | 3.27 × 10−10 | P05549 |
| 4 | TFAP2C | YGCCYBVRGGCA | 4.56 × 10−10 | Q92754 |
| 6 | KLF16 | GMCACGCCCCC | 5.81 × 10−9 | Q9BXK1 |
| Brain | ||||
| 1 | TFAP2A(var.2) | YGCCCBVRGGCR | 1.82 × 10−16 | P05549 |
| 2 | TFAP2B | YGCCCBVRGGCA | 1.29 × 10−13 | Q92481 |
| 3 | SP3 | VCCACGCCCMC | 2.69 × 10−13 | Q02447 |
| 4 | TFAP2C | YGCCYBVRGGCA | 4.99 × 10−13 | Q92754 |
| 5 | KLF16 | GMCACGCCCCC | 1.06 × 10−12 | Q9BXK1 |
| Heart | ||||
| 1 | MZF1 | BGGGGA | 2.23 × 10−5 | P28698 |
| 2 | Ascl2 | ARCAGCTGCY | 7.06 × 10−4 | Q99929 |
| 3 | ASCL1 | VSAGCAGCTGSNN | 9.41 × 10−4 | P50553 |
| 4 | SP3 | VCCACGCCCMC | 1.42 × 10−3 | Q02447 |
| 5 | NEUROD1 | NRACAGATGGYNN | 1.60 × 10−3 | Q13562 |
| Lamina | ||||
| 1 | SP2 | GYCCCGCCYCYBSSS | 8.51 × 10−15 | Q02086 |
| 2 | SP1 | GCCCCKCCCCC | 5.98 × 10−14 | P08047 |
| 3 | SP3 | VCCACGCCCMC | 2.84 × 10−13 | Q02447 |
| 4 | KLF16 | GMCACGCCCCC | 3.22 × 10−13 | Q9BXK1 |
| 7 | Zfx | SSSGCCBVGGCCTS | 1.06 × 10−11 | P17010 |
| Liver | ||||
| 1 | SP1 | GCCCCKCCCCC | 7.81 × 10−13 | P08047 |
| 2 | TFAP2B | YGCCCBVRGGCA | 4.23 × 10−12 | Q92481 |
| 3 | TFAP2C | YGCCYBVRGGCA | 1.87 × 10−11 | Q92754 |
| 4 | TFAP2A | HGCCYSAGGCD | 4.97 × 10−11 | P05549 |
| 5 | ZNF740 | MCCCCCCCAC | 8.99 × 10−11 | Q8NDX6 |
| Lung | ||||
| 1 | THAP1 | YTGCCCDBA | 5.09 × 10−9 | Q9NVV9 |
| 3 | ESR2 | AGGTCASVNTGMCCY | 1.08 × 10−8 | Q92731 |
| 4 | Zfx | SSSGCCBVGGCCTS | 1.44 × 10−8 | P17010 |
| 5 | ZBTB7A | NVCCGGAAGTGSV | 1.46 × 10−8 | O95365 |
| 6 | TFAP2A(var.2) | YGCCCBVRGGCR | 6.51 × 10−8 | P05549 |
| Muscle | ||||
| 1 | SP1 | GCCCCKCCCCC | 1.45 × 10−9 | P08047 |
| 2 | SP2 | GYCCCGCCYCYBSSS | 4.21 × 10−9 | Q02086 |
| 3 | SP8 | RCCACGCCCMCY | 1.15 × 10−8 | Q8IXZ3 |
| 4 | CTCFL | CRSCAGGGGGCRSB | 3.44 × 10−8 | Q8NI51 |
| 5 | KLF16 | GMCACGCCCCC | 4.36 × 10−7 | Q9BXK1 |
| Ovary | ||||
| 1 | FOXO3 1 | DAAAYA | 7.23 × 10−7 | O43524 |
| 3 | KLF16 | GMCACGCCCCC | 1.93 × 10−4 | Q9BXK1 |
| 4 | FOXC1 1 | WAWGTAAAYAW | 2.39 × 10−4 | Q12948 |
| 6 | CTCFL | CRSCAGGGGGCRSB | 4.38 × 10−4 | Q8NI51 |
| 7 | Arid5a | SYAATATTGVDANH | 4.99 × 10−4 | Q03989 |
1 Transcription factor (TF) motifs that were only detected in one tissue.