Figure 4.

IL-37 inhibits caspase-1 activation. (a,b) WT or IL-37tg macrophages were stimulated with LPS (50 ng/mL) as indicated. After 4 h, nigericin (3 μM) was added for another 2 h. Control cells were treated with vehicle. Caspase-1 (pro-caspase-1 p45 and cleaved caspase-1 p20) and β-actin were assessed by immunoblot. Depicted is (a) one representative blot of three independent experiments (lanes irrelevant to this study were cut from the immunoblot images) and (b) the caspase-1 p20 signal intensity quantification normalized to β-actin. Caspase-1 p20 protein abundance is presented as change in caspase-1 p20 compared to WT (set as 100%) ± SEM. *, p < 0.05 for IL-37tg compared to WT. LPS: lipopolysaccharide, tg: transgenic, WT: wild-type.