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. 2020 Jan 10;9(1):178. doi: 10.3390/cells9010178

Figure 5.

Figure 5

IL-37 inhibits pyroptosis. (a,b) WT or IL-37tg macrophages were treated with vehicle or stimulated with LPS (10 ng/mL or 50 ng/mL) as indicated. After 3 h, total RNA was isolated; gene expression was analyzed by real-time PCR and normalized to the abundance of 18S rRNA. Gene expression of Casp4 (a) or Gsdmd (b) is shown as fold-change relative to WT macrophages treated with vehicle for the same duration (set as 1) ± SEM; n = 6. *, p < 0.05 and **, p < 0.01 for LPS versus vehicle-treated cells of the same strain. (c) WT or IL-37tg macrophages were stimulated with LPS as indicated. After 3 h, nigericin was added for another 3 h. Control cells were treated with vehicle. LDH was measured in cell supernatants and LDH release is depicted as fold-change relative to vehicle-treated cells of the same strain (set as 1) ± SEM; n = 5. *, p < 0.05 and ***, p < 0.001 for LPS/nigericin versus vehicle-treated cells of the same strain; ##, p < 0.01 for IL-37tg versus WT macrophages of same treatment group. LPS: lipopolysaccharide, tg: transgenic, WT: wild-type, LDH: lactate dehydrogenase.