Figure 3.

In vitro functional studies after E/R abrogation. (A) Cell cycle distribution of control clones and E/R KO cells at 48 h. (B) Carboxyfluorescein Succinimidyl Ester (CFSE) quantification by flow cytometry after 48 in culture. The peak on the right (10³) represents the percentage of cells that have not divided and the left peak (10²) represents the percentage of cells that have divided and therefore diluted their CFSE expression. (C) CFSE expression by flow cytometry of cells co-cultured with Mesenchymal Stromal Cells (MSC) cell line HS-5 at 48 h. (D) Apoptosis level quantification by Propidium Iodide (PI) expression. The figure shows the percentage of PI negative cells (left) and PI positive cells (right) at 48 h. (E) Apoptosis level quantification by PI expression after treatment with Vincristine (1 μM) at 48 h. On the right is represented the mean distribution of control clones (dark grey) and E/R KO clones (grey) of different experiments. All the experiments were carried out by triplicate. * p ≤ 0.05 (unpaired t-test).