Figure 6.

In vivo effects of CRISPR-mediated editing of the E/R oncogene. (A) External appearance of mice and developed tumours 48–62 days after subcutaneous cell injection. Tumours formed by KO clones (right flank) were smaller than those induced by REH cells or control clones (control c.) (left flank). Each group had four mice. (B) Evolution of tumour growth measured every 2–3 days until the moment in which mice were sacrificed. (C) Representation of the mean tumour size corresponding to each clone, independently of the group. * p ≤ 0.05; ** p ≤ 0.005 (unpaired t-test).