Effects of miR-23b/27b/24 on cell migration. (A,B) Transwell migration (n = 4) assays were performed in mixed mimic or inhibitor of microRNAs (miR-23b, miR-27b and miR-24)-transfected HCT116 cells. Upper panels show representative images of Diff-Quick staining in four experiments of transwell migration assays, and a lower graph shows quantification of cell migration expressed by cell counting. (C–E) Transwell migration assays were performed in mixed mimic of microRNAs (miR-23b, miR-27b, and miR-24)-transfected DLD1, RKO, or HT29 cells. Upper panels and lower graphs are the same as in (aA). Values represent mean ± SD. n = 4. * Statistically significant difference versus control (unpaired Student’s t-test, p < 0.05).