Figure 5.

miR-23b and miR-27b promote cell migration. Transwell assay (A) and Scratch assay (B) were performed in non-MG cells with combinational transfection of mimic miR-23b, miR-27b, and/or miR-24. (A) Upper panels show representative images of Diff-Quick staining in four experiments of transwell migration assays, and a lower graph shows quantification of cell migration expressed by cell counting. Data are expressed as the mean ± standard deviation (SD; n = 4). * Statistically significant difference versus control (unpaired Student’s t-test, p < 0.05). (B) After transfection with mimic miRNAs, cells were scratched. Migration distance was measured at 24 h later. Data are expressed as the mean ± standard deviation (SD; n = 4). * Statistically significant difference versus control (unpaired Student’s t-test, p < 0.05).