Skip to main content
. 2020 Jan 11;9(1):186. doi: 10.3390/cells9010186

Figure 4.

Figure 4

miR-340-3p and miR-132-5p trigger neuronal cell death in co-cultures of neurons and microglia. (a,b) Murine neuron/microglia co-cultures derived from C57BL/6 mice were incubated with 5 μg/mL of mmu-miR-340-3p, mmu-miR-132-5p, or control mutant oligoribonucleotide (ctrl oligo) for 3 days. LPS (100 ng/mL) and loxoribine (1 mM) were used as positive control, while LyoVec and unstimulated condition served as negative control. Subsequently, cells were stained with NeuN antibody and DAPI. (a) Relative neuronal viability was assessed by quantification of NeuN-positive cells. Significances were obtained by Student’s t-Test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; * compared to unstimulated control. Data of n = 3 are represented as mean ± s.e.m. (b) Representative images of neuron/microglia co-cultures from C57BL/6 mice immunostained as described above. Scale bar, 20 µm.