Expression of miR-9-5p and methylation of miR-9 precursors in cervical tissue samples. (a) Expression was determined by microarray analysis in micro-dissected SCCs (n = 10) and ACs (n = 9) [8]; (b) miR-9-5p expression was stratified by the presence (seven SCCs, four ACs) or absence (three SCCs, three ACs) of a 1q gain. For two ACs chromosomal data was not available; (c) methylation levels of miR-9-5p precursors miR-9-1, miR-9-2, and miR-9-3 were determined by quantitative MSP in 24 SCC and 17 AC tissue samples; (d) methylation analysis was further stratified by hrHPV type (HPV16 or other). HPV16-positive carcinomas included 11 SCCs and eight ACs. Carcinomas positive for non-HPV16 types were comprised of 13 SCCs (of which one was positive of HPV18) and nine ACs (of which seven were positive for HPV18). SCC: Squamous cell carcinoma; AC: Adenocarcinoma. * p < 0.05, ** p < 0.005.