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. 2019 Dec 26;9(1):65. doi: 10.3390/cells9010065

Figure 4.

Figure 4

miR-9-5p directly targets transcription factor twist family bHLH transcription factor 1 (TWIST1). (a) Endogenous expression of TWIST1 mRNA in cervical cancer cell lines SiHa, CaSki, and HeLa; (b) binding site of miR-9-5p in the TWIST1-3UTR with the seed sequence indicated in red. Dual-luciferase reporter assay in HEK293 cells transiently transfected with a negative control or miR-9-5p mimics in combination with either an empty pmiRGLO vector (-), a pmiRGLO-TWIST1-UTR construct (TWIST1_UTR) or a pmiRGLO-TWIST1_UTR construct with mutated binding site of miR-9-5p (TWIST1-UTR_mut); (c) TWIST1 mRNA expression upon inhibition and overexpression of miR-9-5p relative to the respective negative control of TWIST1 mRNA in cervical cancer cell lines SiHa, CaSki, and HeLa. * p < 0.05, ** p < 0.005.