Table 1.
Intermolecular cross-links identified between CaM and the bMunc13-2 segment-A at 750 nM and 1 mM Ca2+. x denotes photo-Met; Y, cross-link detected; N, cross-link not detected.
| Cross-Linker | Cross-Linked Amino Acid CaM | Cross-Linked Amino Acid Segment-A | Ca2+ Concentration | |
|---|---|---|---|---|
| 750 nM | 1 mM | |||
| Photo-Met | x36 | D615 | N | Y |
| x36 | E712 | Y | Y | |
| x71/x72 | E707 | Y | Y | |
| x71/x72 | E740 | N | Y | |
| x76 | H578 | Y | Y | |
| x76 | E707 | Y | Y | |
| x76 | E729 | N | Y | |
| x144/x145 | H578/H579 | Y | Y | |
| x144/x145 | E707 | N | Y | |
| x144/x145 | Y715 | Y | Y | |
| x144/x145 | C719 | Y | Y | |
| x144/x145 | E729 | Y | Y | |
| DSBU | K75 | K735 | N | Y |
| s-GMBS | K75 | C453 | N | Y |
| K13 | C428 | N | Y | |
| K30 | C428 | N | Y | |