Table 2.
Comparison of intermolecular cross-links identified between CaM and bMunc13-2 segments. The constraints derived from the cross-links with segment-A and segment-B were used for structural modeling. x denotes photo-Met.
| Cross-Linked Amino Acid CaM | Cross-Linked Amino Acid bMunc13-2 | Segment-A (aa 367–780) | Segment-B (aa 367–903) | Segment-C (aa 703–742) |
|---|---|---|---|---|
| x36 | E707 | ✓ | ||
| x36 | D615 | ✓ | ||
| x36 | E712 | ✓ | ✓ | |
| x71/x72 | E707 | ✓ | ✓ | ✓ |
| x71/x72 | E729 | ✓ | ||
| x71/x72 | E740 | ✓ | ✓ | |
| x76 | H578 | ✓ | ||
| x76 | E707 | ✓ | ✓ | ✓ |
| x76 | E729 | ✓ | ✓ | |
| x124 | E729 | ✓ | ||
| x144/x145 | H578/H579 | ✓ | ||
| x144/x145 | E707 | ✓ | ✓ | ✓ |
| x144/x145 | Y715 | ✓ | ✓ | ✓ |
| x144/x145 | C719 | ✓ | ✓ | ✓ |
| x144/x145 | E729 | ✓ | ✓ |