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. 2020 Jan 12;9(1):193. doi: 10.3390/cells9010193

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Age-induced changes in visfatin expression in dental pulp tissues. (a–d) Representative microphotographs of sections of dental pulp stained with anti-visfatin antibodies from individuals aged 17 (a), 20 (b), 35 (c), and 45 (d) years old. The number of visfatin-positive cells increased significantly in the Old group than in the Young group. All microphotographs were taken at an original magnification of × 200. Scale bar: 50 μm. (e) Bar graph illustrating the age-related changes in visfatin immunoexpression in the human dental pulp. Kruskal–Wallis test was performed to analyze the difference in number of visfatin-positive cells between Young group and Old group. # p < 0.0001.