Figure 5.

FK866 impedes the visfatin-induced senescence of human dental pulp cells (hDPCs). hDPCs were pretreated with FK866 (10 µM) for 2 h and then incubated with visfatin (500 ng/mL) for 24 h. (a) Cell extracts were subjected to Western blotting to detect p53 and p21 proteins. α-Tubulin was used as the loading control. (b) Densitometric analysis for assessing relative p21 protein levels were normalized to α-Tubulin protein levels. (c) Representative image of senescence-associated (SA)-β-galactosidase staining. Scale bar: 200 μm. (d) Quantification of the percentage of SA-β-galactosidase-positive cells. ** p < 0.01, *** p < 0.001.