Figure 2.

TNS3 is involved in cell cycle regulation and the pluripotency of TMSCs. After siTNS3 treatment, p16 was increased, and SOX2 was inversely decreased in both RT-qPCR (A) and Western blotting assays (B). Extracellular signal-regulated kinase (ERK), protein kinase B (AKT), and c-Jun N-terminal kinase (JNK) levels were all decreased after siTNS3 treatment, with that of ERK being lower (B). (C) For cell cycle analyses, 6-well plates were seeded with 5 × 10⁴ cells/well and analyzed with the FACS Calibur system. The proportion of active phase cells (S + G2/M) was reduced, and that in the G1/G0 phase was increased. *, p < 0.05 compared to the control. n.s., not significant compared to the control. n = 3. Columns and error bars represent the mean ± standard deviation.