Figure 3.
TNS3 is involved in regulating TMSC senescence. (A) Beta-galactosidase staining and colony-forming unit assay were performed in early, intermediate, and late passages of TMSCs. (B) MTT assay was conducted to evaluate the proliferation of TMSCs at different passages. (C) Protein expression of TNS3, SOX2, p16, and p21 was detected in TMSCs at different passages by Western blotting. *, p < 0.05 compared to the control. n = 3. Columns and error bars represent the mean ± standard deviation.