Table 2. Result of Sequential Extraction and Isomerization Using Naphthalne-2-boronic Acid as the Complexing Agent.
| aq. phase isomerizationa |
org. extractionb |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| run | [Glu]0 | [Fru]0 | [Man]0 | [Psi]0 | [Glu] | [Fru] | [Man] | [Psi] | RFru (%) | RMan (%) | RPsi (%) | RGlu (%) | total RFru (%) | total RGlu (%) | overall isomerization YFruc (%) |
| I | 100 | 0 | 0 | 0 | 55 | 30 | 4.93 | 4.3 | 24.19 | 15.52 | 33.01 | 2.59 | |||
| 14.52 | 11.82 | 24.88 | 3.19 | ||||||||||||
| 14.43 | 10.29 | 12.44 | 3.97 | ||||||||||||
| 7.67 | 8.57 | 10.37 | 4.47 | 60.82 | 14.23 | 30 | |||||||||
| II | 88 | 12 | 0 | 54 | 32 | 4.93 | 4.12 | 21.56 | 13.91 | 25.37 | 2.44 | ||||
| 14.73 | 10.78 | 17.55 | 3.09 | ||||||||||||
| 11.28 | 5.58 | 12.56 | 3.96 | ||||||||||||
| 9.40 | 7.92 | 9.18 | 5.37 | 58.79 | 18.84 | 53 | |||||||||
| III | 85 | 15 | 0 | 0 | 52 | 34 | 4.93 | 4.3 | 21.56 | 13.91 | 21.56 | 1.96 | |||
| 18.07 | 10.78 | 15.65 | 2.98 | ||||||||||||
| 11.06 | 5.58 | 12.30 | 3.86 | ||||||||||||
| 9.40 | 7.92 | 9.18 | 4.07 | 57.20 | 23.33 | 75 | |||||||||
a
Isomerization conditions: 6% spermine dose at 100 °C for 15 min.
b
Extraction conditions: 2 mL of solution containing 3% mol of spermine, fructose and glucose (aqueous phase), and 2 mL of solution containing 0.1 M NBA, 0.4 M Aliquat 336, 1-octanol (organic phase) were shaken together for 1 h at room temperature. Rx (calculated as the difference in the concentration of monosugar before and after extraction) and Yx is the yield of sugars, where x can be glucose, fructose, mannose, and psicose.