Table 2. ADP Binding Constants Determined by Fitting to Two-Dimensional Lineshapes.

protein–ligand	KD (μM)	koff (s–1)
WT–TMP	15 ± 2.1	12 ± 0.7
WT–AZTMP	15 ± 1.3	24 ± 0.8
F105Y–TMP	4.2 ± 1.3	2.7 ± 0.8
F105Y–AZTMP	4.4 ± 1.7	0.20 ± 0.9