Figure 1.
Percentage of samples quantifiable for adenovirus (AdV), norovirus genogroup I (NoVGI), and NoVGII in three decentralized combined wastewater (WW1) and graywater (GW1 and GW2) collection systems by quantitative polymerase chain reaction (qPCR) and droplet digital PCR (ddPCR). Also shown are putative detections below the lower limit of quantification (LOQ) and non-detections below the limit of detection (LOD). WW-Sim and GW-Sim columns present expected results based on previously published epidemiology-based simulations of these pathogens in comparably sized collection systems,11 i.e. 1000-person for combined wastewater (WW-Sim 1000P) and 100- or 1000-person for graywater (GW-Sim 100P and GW-Sim 1000P), where bars represent the percentage of daily simulations (n=365×10,000) that would fall within each category given respective LOQs for each PCR assay. Since the simulation did not distinguish between norovirus genogroups, expected results for NoVGII represent NoVGI and NoVGII combined.